NEO 500 experimental setups
NEO 500 MHz
Experimental setups
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| Experiment | Pulse sequence | Parameter set | comments |
| 1D proton | |||
| simple acquisition | zg | itqb_1d1h | |
| pulse calibration | zg490.rak | itqb_1d1h_pulse | |
| pre-saturation | zgpr | itqb_1d1h_presat | adjust O1 |
| Excit sculpting | zgesgp | itqb_1d1h_es | needs correct 90°Pulse and O1 |
| watergate | zggpwg | itqb_1d1h_wg | adjust sp1 with gs |
| binomial watergate | p3919gp | itqb_1d1h_3919 | adjust d19 for next null and p28 with gs |
| double binomial watergate | zgpgpw5 | itqb_1d1h_w5 | adjust d19 for next null and p28 with gs |
| NOE w/pre-saturation | noesypr1d | itqb_1d1h_noepr | needs correct 90°Pulse and O1 |
| NOE w/ pre-saturation and grad | noesygppr1d | itqb_1d1h_noegppr | needs correct 90°Pulse and O1 |
| 1H T1 measur 1D | t1ir1dpr.pl | itqb_1d1h_T1pr | needs 90°Pulse. variable D7, long D1 |
| 1H-1H 2D | |||
| COSY presat | cosygpppqfpr.pl | itqb_2d1h_cosygppr | needs correct 90°Pulse and O1 |
| DQF-COSY with exc. sculp. | cosydfesgpph | itqb_2d1h_cosydqesgp | needs correct 90°Pulse and O1 |
| NOESY presat | noesygpphpr | itqb_2d1h_noesygppr | d8 is mix time, be careful with RGA |
| NOESY w/es | noesyesgpph | itqb_2d1h_noesyesgp | d8 is mix time, be careful with RGA |
| ROESY w/es | roesyesgpph | itqb_2d1h_roesyes | p15 is mixing time |
| TOCSY w/es (mlev) | mlevesgpph | itqb_2d1h_mlevesgp | d9-mixing time |
| TOCSY w/es (dipsi2) | dipsi2esgpph | itqb_2d1h_dipsies | d9-mixing time |
| J-resolved w/pr | jresgsprqf | itqb_2d1h_jrespr | |
| 1H-13C 2D | |||
| HSQC w/ adiab. pulses and presat | hsqcetgpsisp2.2pr.pl | itqb_2d13C_hsqcsipr | |
| HSQC w/ multiplicity editing presat | hsqcedetgpsisp2.2pr.pl | itqb_2d13C_hsqcedpr | |
| HSQC-TOCSY w/ adiab. pulses | hsqcdietgpsisp2.2 | itqb_2d13C_hsqctoscsy | d9-mixing time |
| HMBC double lowpas presat | hmbcetgpl2ndpr.pl | itqb_2d13C_hmbcl2pr | |
| HMBC triple lowpas presat | hmbcetgpl3ndpr.pl | itqb_2d13C_hmbcl3pr | |
| HMQC w/presat | hmqcetgpsi.2pr.pl | itqb_2d13C_hmqcpr | |
| 1H-15N 2D | |||
| HSQC f3 | hsqcetf3gpsi | itqb_2d15N_hsqcf3 | |
| HSQC w/ adiab. pulses and presat | hsqcetgpsisp2.2pr.pl | itqb_2d15N_hsqcsipr | |
| HSQC-TOCSY w/ adiab. pulses | hsqcdietgpsisp2.2 | itqb_2d15N_hsqctocsysi | d9-mixing time |
| Sofast HSQC | sfhmqcf3gpph | itqb_2d15N_sfhmqc |
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| Experiment | Pulse sequence | Parameter set | comments |
| 1D Carbon/Fluoride | |||
| 13C non-dec | zg | itqb_1d13C_zg | |
| 13C w dec | zgig | itqb_1d13C_zgig | |
| 13C w dec and NOE | zgdc | itqb_1d13C_zgdc | |
| 13C non-dec w NOE | zggd | itqb_1d13C_zggd | |
| 13C APT | jmod | itqb_1d13C_apt | |
| 19F non-dec | zg | itqb_1d19F_zg | mind the SW and O1 |
| 1H-1H 2D | |||
| Magnitude COSY | cosygpppqf | itqb_2d1h_cosymag | do not trust RGA |
| DQF-COSY | cosygpmfphpp | itqb_2d1h_cosydqf | do not trust RGA |
| ROESY | roesyphpp.2 | itqb_2d1h_roesy | p15 is mix time |
| NOESY | noesygpphpp | itqb_2d1h_noesy | d8 is mix time |
| TOCSY | mvelphpp | itqb_2d1h_tocsy | d9 is mixing time |
| J-resolved | jresgpqf.pl | itqb_2d1h_jres | |
| 1H-13C 2D | |||
| HSQC w/ adiab. pulses | hsqcetgpsisp2.2 | itqb_2d13C_hsqcadia | |
| HSQC w/ multiplicity editing | hsqcedetgpsisp2.2 | itqb_2d13C_hsqced | |
| HSQC-TOCSY w/ adiab. pulses | hsqcdietgpsisp2.2 | itqb_2d13C_hsqctocsy | d9 is mixing time |
| HMBC double lowpas | hmbcetgpl2nd | itqb_2d13C_hmbc2 | |
| HMBC triple lowpas | hmbcetgpl3nd | itqb_2d13C_hmbc3 | |
| H2BC | h2bcetgpl3 | itqb_2d13C_h2bc | |
| 1H-15N 2D | |||
| HSQC f3 | hsqcetf3gpsi | itqb_2d15N_hsqcf3.pl | |
| HSQC w/ adiab. pulses | hsqcetgpsisp2.2 | itqb_2d15N_hsqcsi.pl | |
| HSQC-TOCSY w/ adiab. pulses | hsqcdietgpsisp2.2 | itqb_2d15N_hsqctocsysi.pl | d9-mixing time |
| Sofast HSQC | sfhmqcf3gpph | itqb_2d15N_sfhmqc.pl | |
| 19F-13C 2D | |||
| HSQC | hsqcetgpsi2 | itqb_2d19F13C_hsqc | |
| HMBC | hmbcetgpl3nd | itqb_2d19F13C_hmbc |