In vivo NMR
A subject with a long history and tradition at ITQB, in vivo NMR of Bacteria and immobilized eukaryotic cells has been performed at CERMAX to study in vivo metabolism and metabolic fluxes using a specific set-up and uniformly or site specific 13C labeled compounds
Experimental set-up used for in vivo NMR of cell suspensions
Example of a time series of 13C spectra of a cell suspension of a strain of L. lactis after a pulse of glucose labelled specifically at carbon 1.