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In vivo NMR

A subject with a long history and tradition at ITQB, in vivo NMR of Bacteria and immobilized eukaryotic cells has been performed at CERMAX to study in vivo metabolism and metabolic fluxes using a specific set-up and uniformly or site specific 13C labeled compounds

Experimental set-up used for in vivo NMR of cell suspensions

Example of a time series of 13C spectra of a cell suspension of a strain of L. lactis after a pulse of glucose labelled specifically at carbon 1.